Details

Autor(en) / Beteiligte

• Zimpfer, Mark
• Mizer, Linda A.
• Weisbrode, Steven E.
• Martich, Daniel
• Dorinsky, Paul M.

Titel

Oxygen uptake-oxygen delivery alterations in the isolated liver after hydrogen peroxide challenge

Ist Teil von

• Journal of critical care, 1993-03, Vol.8 (1), p.24-33

Ort / Verlag

Philadelphia, PA: Elsevier Inc

Erscheinungsjahr

1993

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Acute, diffuse lung injury is frequently complicated by systemic organ injury and alterations in the relationship between oxygen uptake (VO 2) and oxygen delivery (QO 2). In this regard, systemic organ neutrophil accumulation and morphologic alterations consistent with systemic organ injury often occur in nonpulmonary organs in these settings. However, whether VO 2-QO 2 matching is also altered in these injured systemic organs remains unproven. Thus, the present study was designed to test the hypothesis that hydrogen peroxide (H 2O 2), a product of neutrophil oxidative metabolism, will cause systemic organ structural abnormalities and alter VO 2-QO 2 matching. To test this hypothesis, VO 2-QO 2 relationships, morphologic changes, and organ water content were evaluated in both uninjured, isolated perfused rabbit livers and in isolated perfused rabbit livers after injury with 5 mmol/L H 2O 2. Following H 2O 2 injury, peak VO 2 fell from 1.36 ± 0.35 mL/min/ 100 g to 0.79 ± 0.16 mL/min/ 100 g ( P <.05) and peak O 2 extraction fell from 0.83 ± 0.09 to 0.66 ± 0.04 ( P < .05). In addition, VO 2 was lower for any given level of QO 2 in the H 2O 2-injured livers compared with the control livers ( P < .01). Finally, liver extravascular water content was increased in H 2O 2-injured livers compared with the control livers (0.79 ± 0.02 v 0.71 ± 0.05; P <.05). These observations indicate that H 2O 2, a product of neutrophil oxidative metabolism, is capable of producing both morphologic changes as well as gas exchange alterations in the isolated, perfused liver.