Details

Autor(en) / Beteiligte

• Goodwin, T. J.
• Prewett, T. L.
• Wolf, David A.
• Spaulding, G. F.

Titel

Reduced shear stress: A major component in the ability of mammalian tissues to form three-dimensional assemblies in simulated microgravity

Ist Teil von

• Journal of cellular biochemistry, 1993-03, Vol.51 (3), p.301-311

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

1993

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• BHK‐21 cells were cultured under various shear stress conditions in an Integrated Rotating‐Wall Vessel (IRWV). Shear ranged from 0.5 dyn/cm2 (simulated microgravity) to 0.92 dyn/cm2. Under simulated microgravity conditions, BHK‐21 cells complexed into three‐dimensional cellular aggregates attaining 6 × 106 cells/ml as compared to growth under 0.92 dyn cm2 conditions. Glucose utilization in simulated microgravity was reduced significantly, and cellular damage at the microcarrier surface was kept to a minimum. Thus, the integrated rotating wall vessel provides a quiescent environment for the culture of mammalian cells. © 1993 Wiley‐Liss, Inc.