Details

Autor(en) / Beteiligte

• Zenzmaier, Christoph
• Kollroser, Manfred
• Gesslbauer, Bernd
• Jandrositz, Anita
• Preisegger, Karl-Heinz
• Kungl, Andreas J

Titel

Preliminary 2-D chromatographic investigation of the human stem cell proteome

Ist Teil von

• Biochemical and biophysical research communications, 2003-10, Vol.310 (2), p.483-490

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• Stem cells represent a promising tool for the treatment of various hematopoietic diseases. In order to identify stem cell-specific proteins, the proteome of human stem cells from umbilical cord blood was explored for the first time. For this purpose, the crude lysate of 4 × 10 5 CD34+ cells was subjected to in solution trypsin digestion. The resulting peptides were then separated via cation exchange followed by reversed phase chromatography and analyzed by nanospray MS/MS. Database search revealed a total of 215 proteins which could be reliably identified. To obtain a more complete picture of the human stell cell proteome and to also access low abundant proteins, pooling of more than one CD34+ preparations seems necessary in order to increase the cell number and thus the protein content.