Details

Autor(en) / Beteiligte

• Huiet, Layne
• Tsai, James H
• Falk, Bryce W

Titel

Maize stripe virus RNA5 is of negative polarity and encodes a highly basic protein

Ist Teil von

• Journal of general virology, 1993-04, Vol.74 (4), p.549-554

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

1993

Quelle

MEDLINE

Beschreibungen/Notizen

• 1 Department of Plant Pathology, University of California, Davis, California 95616 and 2 University of Florida, Fort Lauderdale Research and Education Center, Fort Lauderdale, Florida 33314, U.S.A. The entire 1317 nucleotide sequence of the maize stripe virus (MStV) RNA5 was determined. Only one open reading frame (ORF) was identified and was found in the viral complementary RNA (vcRNA). This ORF appears to encode a protein of M r 44237, hereafter referred to as NS 5 . In vitro translation of transcripts representing nearly full-length RNA5 vcRNA yielded products of the predicted size, as well as some smaller, less prominent products. No products were identified from transcripts of viral polarity. RNA hybridization analyses of MStV-infected Zea mays revealed RNAs corresponding only to full-length RNA5, but both positive and negative polarity RNAs were abundant. Analysis of the NS 5 amino acid sequence revealed that it is extremely basic, containing 21% arginine and lysine. Database comparisons showed that NS 5 had no significant similarity to other protein sequences. Present address: Centre for Engineering Plants for Resistance Against Pathogens (CEPRAP), 1930 5th Street, University of California, Davis, California 95616, U.S.A. Received 9 October 1992; accepted 8 December 1992.