Details

Autor(en) / Beteiligte

• LaPolla, R.J
• Lambowitz, A.M

Titel

Mitochondrial ribosome assembly in Neurospora crassa [Fungi]. Purification of the mitochondrially synthesized ribosomal protein, S-5

Ist Teil von

• The Journal of biological chemistry, 1981-07, Vol.256 (13), p.7064-7067

Ort / Verlag

United States: American Society for Biochemistry and Molecular Biology

Erscheinungsjahr

1981

Quelle

MEDLINE

Beschreibungen/Notizen

• In Neurospora, one mitochondrial ribosomal protein (S-5, Mr = 52,000) is synthesized intramitochondrially and is presumably encoded by mitochondrial DNA. We have developed a rapid method for the purification of S-5 which takes advantage of its high affinity for carboxymethyl-Sepharose in the presence of 6 M urea. Using this method, S-5, at purity greater than 95%, can be prepared by column chromatography in a single batch elution step. The amino acid composition of S-5 was determined. Judged by the contents of hydrophilic and basic amino acids, S-5 is more similar to Escherichia coli and yeast ribosomal proteins than to other mitochondrial translation products which are hydrophobic membrane proteins.