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Mitochondrial ribosome assembly in Neurospora crassa [Fungi]. Purification of the mitochondrially synthesized ribosomal protein, S-5
Ist Teil von
The Journal of biological chemistry, 1981-07, Vol.256 (13), p.7064-7067
Ort / Verlag
United States: American Society for Biochemistry and Molecular Biology
Erscheinungsjahr
1981
Quelle
MEDLINE
Beschreibungen/Notizen
In Neurospora, one mitochondrial ribosomal protein (S-5, Mr = 52,000) is synthesized intramitochondrially and is presumably
encoded by mitochondrial DNA. We have developed a rapid method for the purification of S-5 which takes advantage of its high
affinity for carboxymethyl-Sepharose in the presence of 6 M urea. Using this method, S-5, at purity greater than 95%, can
be prepared by column chromatography in a single batch elution step. The amino acid composition of S-5 was determined. Judged
by the contents of hydrophilic and basic amino acids, S-5 is more similar to Escherichia coli and yeast ribosomal proteins
than to other mitochondrial translation products which are hydrophobic membrane proteins.