Details

Autor(en) / Beteiligte

• Ulmer, Jeffrey B.
• Donnelly, John J.
• Parker, Suezanne E.
• Rhodes, Gary H.
• Felgner, Philip L.
• Dwarki, V. J.
• Gromkowski, Stanislaw H.
• Deck, R. Randall
• DeWitt, Corrille M.
• Friedman, Arthur
• Hawe, Linda A.
• Leander, Karen R.
• Martinez, Douglas
• Perry, Helen C.
• Shiver, John W.
• Montgomery, Donna L.
• Liu, Margaret A.

Titel

Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral Protein

Ist Teil von

• Science (American Association for the Advancement of Science), 1993-03, Vol.259 (5102), p.1745-1749

Ort / Verlag

Washington, DC: American Society for the Advancement of Science

Erscheinungsjahr

1993

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Cytotoxic T lymphocytes (CTLs) specific for conserved viral antigens can respond to different strains of virus, in contrast to antibodies, which are generally strain-specific. The generation of such CTLs in vivo usually requires endogenous expression of the antigen, as occurs in the case of virus infection. To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleoprotein was injected into the quadriceps of BALB/c mice. This resulted in the generation of nucleoprotein-specific CTLs and protection from a subsequent challenge with a heterologous strain of influenza A virus, as measured by decreased viral lung titers, inhibition of mass loss, and increased survival.