Details

Autor(en) / Beteiligte

• Dubois, Madeleine
• Picq, Madeleine
• Némoz, Georges
• Lagarde, Michel
• Prigent, Annie-France

Titel

Inhibition of the Different Phosphodiesterase Isoforms of Rat Heart Cytosol by Free Fatty Acids

Ist Teil von

• Journal of cardiovascular pharmacology, 1993-04, Vol.21 (4), p.522-529

Ort / Verlag

Philadelphia, PA: Lippincott-Raven Publishers

Erscheinungsjahr

1993

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The sensitivity of the various phosphodiesterase (PDE) isoforms present in the cytosolic compartment of rat heart to the main fatty acids of the saturated, n-3 and n-6 families was assessed. High-performance liquid chromatography (HPLC) on a Mono Q ion-exchange column resolved four separate cyclic nucleotide phosphodiesterase activitiesa calmodulin-activated fraction, a cyclic GMP-stimulated fraction, a cyclic AMP-specific rolipram-sensitive fraction, and a cyclic GMP-inhibited fraction. Polyunsaturated fatty acids (PUFA) were more potent inhibitors than saturated fatty acids whatever the considered PDE isoform. Although all PDE isoforms were affected, the cyclic GMP-stimulated isoform was the most sensitive to the inhibitory effect of PUFAs. The possible influences of free fatty acids (FFA) on cardiac contractility through PDE inhibition are discussed.