Details

Autor(en) / Beteiligte

• E E Max
• J V Maizel, Jr
• P Leder

Titel

The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes

Ist Teil von

• The Journal of biological chemistry, 1981-05, Vol.256 (10), p.5116-5120

Ort / Verlag

United States: American Society for Biochemistry and Molecular Biology

Erscheinungsjahr

1981

Quelle

MEDLINE

Beschreibungen/Notizen

• We have determined the nucleotide sequence of a 5.5-kilobase segment of cloned mouse DNA which includes regions encoding two parts of the mouse kappa immunoglobulin gene: the J regions (amino acids 96-108 of the kappa chain) and the C region (residues 109-214). This sequence allows us to rule out interruptions in the germline constant region coding segment as well as the presence of additional functional J genes in the sequenced DNA segment, although two weak homologies to J regions have been found. The complete sequence also allows us to identify a single occurrence of the heptanucleotide palindrome thought to play a role in V/J joining. This palindrome, midway between J and C regions, is the site of aberrant joining in the plasmacytoma MPC11 and may be a target for such aberrant recombination of other kappa genes. In addition, computer analysis of the J sequences suggests that those closest to the C region arose by the most recent duplication event.