Analytical biochemistry, 1993-02, Vol.208 (2), p.270-276
1993
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Details
- Autor(en) / Beteiligte
- Titel
- Purification of Interferon γ-Interferon γ Receptor Complexes by Preparative Electrophoresis on Native Gels
- Ist Teil von
- Analytical biochemistry, 1993-02, Vol.208 (2), p.270-276
- Ort / Verlag
- San Diego, CA: Elsevier Inc
- Erscheinungsjahr
- 1993
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- Cytokine-receptor complexes are required for certain studies including crystallization, NMR spectra, and investigation of the biological response mechanism to the cytokine. The purity of the ligand-receptor complex is critical for most of these applications. We investigated the possibility of purifying protein-protein complexes by electrophoresis on native gels. Starting with partially purified mouse and highly purified human proteins, we prepared milligram amounts of interferon γ-interferon γ receptor complexes by preparative electrophoresis on nondenaturing polyacrylamide gels. In both cases, pure ligand-receptor complexes with the correct stoichiometry of binding were recovered. Electrophoresis on preparative native gels may prove to be of general interest for the preparation of protein-protein complexes to be used in diverse studies.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0003-2697eISSN: 1096-0309DOI: 10.1006/abio.1993.1045Titel-ID: cdi_proquest_miscellaneous_75617469
- Format
- –
- Schlagworte
- Amino Acids - analysis, Animals, Biological and medical sciences, complexes, Electrophoresis, Polyacrylamide Gel - methods, Evaluation Studies as Topic, Fundamental and applied biological sciences. Psychology, Fundamental immunology, gamma -interferon, gel electrophoresis, gels, Humans, In Vitro Techniques, Interferon gamma Receptor, Interferon-gamma - chemistry, Interferon-gamma - isolation & purification, Interferon-gamma - metabolism, man, Mice, Molecular immunology, Molecular Weight, native, Protein Binding, Protein Conformation, purification, receptors, Receptors, Interferon - chemistry, Receptors, Interferon - isolation & purification, Receptors, Interferon - metabolism, Recombinant Proteins - chemistry, Recombinant Proteins - isolation & purification, Recombinant Proteins - metabolism, Spectrophotometry, Ultraviolet, Techniques