Molecular reproduction and development, 1993-02, Vol.34 (2), p.117-126
1993
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- Autor(en) / Beteiligte
- Titel
- Cytoplasmic β-actin promoter produces germ cell and preimplantation embryonic transgene expression
- Ist Teil von
- Molecular reproduction and development, 1993-02, Vol.34 (2), p.117-126
- Ort / Verlag
- Hoboken: Wiley Subscription Services, Inc., A Wiley Company
- Erscheinungsjahr
- 1993
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The cytoplasmic β‐actin promoter, commonly used as strong promoter in many gene regulation studies, produces a pattern of male germ cell and preimplantation, embryonic gene expression in transgenic mice. In seven of ten expressing transgenic lines, a chicken β‐actin‐lacZ fusion gene was expressed in adult testes. In addition, five of the ten lines demonstrated transgene expression in the preimplantation mouse embryo. This is the first example of transgene expression at the stages of both gamete and early embryo. Overall, the site or transgene integration appeared to influence transgene expression in adult tissues. © 1993 Wiley‐Liss, Inc.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1040-452XeISSN: 1098-2795DOI: 10.1002/mrd.1080340202Titel-ID: cdi_proquest_miscellaneous_75612691
- Format
- –
- Schlagworte
- Actins - genetics, Animals, Beta-galactosidase, beta-Galactosidase - genetics, Biological and medical sciences, Blastocyst - metabolism, Cells, Cultured, Chicken-β-actin, Chickens - genetics, Embryology: invertebrates and vertebrates. Teratology, Fibroblasts, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation, Genes, Synthetic, LacZ, Male, Mice, Mice, Inbred ICR, Mice, Transgenic, Molecular embryology, Organ Specificity, Promoter Regions, Genetic, Spermatozoa - metabolism, Testis, Testis - metabolism, Transgenic mice