Details

Autor(en) / Beteiligte

• Cogliati, Clelia
• Ragona, Laura
• D'Onofrio, Mariapina
• Günther, Ulrich
• Whittaker, Sara
• Ludwig, Christian
• Tomaselli, Simona
• Assfalg, Michael
• Molinari, Henriette

Titel

Site-Specific Investigation of the Steady-State Kinetics and Dynamics of the Multistep Binding of Bile Acid Molecules to a Lipid Carrier Protein

Ist Teil von

• Chemistry : a European journal, 2010-10, Vol.16 (37), p.11300-11310

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

2010

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The investigation of multi‐site ligand–protein binding and multi‐step mechanisms is highly demanding. In this work, advanced NMR methodologies such as 2D 1H–15N line‐shape analysis, which allows a reliable investigation of ligand binding occurring on micro‐ to millisecond timescales, have been extended to model a two‐step binding mechanism. The molecular recognition and complex uptake mechanism of two bile salt molecules by lipid carriers is an interesting example that shows that protein dynamics has the potential to modulate the macromolecule–ligand encounter. Kinetic analysis supports a conformational selection model as the initial recognition process in which the dynamics observed in the apo form is essential for ligand uptake, leading to conformations with improved access to the binding cavity. Subsequent multi‐step events could be modelled, for several residues, with a two‐step binding mechanism. The protein in the ligand‐bound state still exhibits a conformational rearrangement that occurs on a very slow timescale, as observed for other proteins of the family. A global mechanism suggesting how bile acids access the macromolecular cavity is thus proposed. Stuck together: Line‐shape analysis provides a direct view of the mechanism and the kinetic rate constants for the binding events in a complex macromolecular system (see figure). Two probes (1H, 15N) provide information on the effect of binding along the entire titration pathway. The two neighbouring nuclei experience the same kinetic mechanism, overcoming a possible lack of resolution in one dimension.