UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 20 von 66
Datensatz exportieren als...
BibTeX
Discrimination between UV radiation-induced and thermally induced spectral changes in AT-paired DNA oligomers using UV resonance Raman spectroscopy
Journal of Raman spectroscopy, 2006-12, Vol.37 (12), p.1368-1380
Jirasek, Andrew
Schulze, H. Georg
Hughesman, Curtis H.
Creagh, A. Louise
Haynes, Charles A.
Blades, Michael W.
Turner, Robin F. B.
2006
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Jirasek, Andrew
Schulze, H. Georg
Hughesman, Curtis H.
Creagh, A. Louise
Haynes, Charles A.
Blades, Michael W.
Turner, Robin F. B.
Titel
Discrimination between UV radiation-induced and thermally induced spectral changes in AT-paired DNA oligomers using UV resonance Raman spectroscopy
Ist Teil von
Journal of Raman spectroscopy, 2006-12, Vol.37 (12), p.1368-1380
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2006
Quelle
Wiley-Blackwell subscription journals
Beschreibungen/Notizen
Ultraviolet resonance Raman spectroscopy (UVRRS) was used to monitor UV radiation and thermal energy deposition in 12‐ and 18‐mer AT oligomers. Difference spectroscopy and two‐dimensional correlation spectroscopy (2DCOS) were used to characterize the distinct spectral responses manifested by the two processes, and to examine their potential cooperative and/or independent effects on the ensemble spectrum. Experiments utilizing incremental doses of 257‐nm radiation revealed that the most affected bands are those involving double bonds when the sample temperature was held constant. Complementary experiments utilizing sample heating indicated that the most affected bands were those involving hydrogen‐bond disruption. Finally, it is shown that bands associated with Watson–Crick hydrogen‐bond disruption are the most affected in rapidly heat‐cycled samples. The results also suggest that both radiation and thermal effects produce independent structural changes in AT oligomers and, furthermore, that these effects are complementary when the experiments involve single‐strand DNA fragments. When using UVRRS to study one of these perturbations (e.g. thermal stability of DNA), the concurrent perturbation (e.g. UV exposure) must not be neglected, since UV exposure is inherent in the UVRR process. These findings thus have implications for the use of UVRRS in the study of DNA dynamics. Copyright © 2006 John Wiley & Sons, Ltd.
Sprache
Englisch
Identifikatoren
ISSN: 0377-0486
eISSN: 1097-4555
DOI: 10.1002/jrs.1552
Titel-ID: cdi_proquest_miscellaneous_754879028
Format
–
Schlagworte
2D correlation spectroscopy
,
DNA
,
oligomer nucleotide
,
UV resonance Raman spectroscopy
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX