Details

Autor(en) / Beteiligte

• Adjei, M. D
• Deck, J
• Heinze, T. M
• Freeman, J. P
• Williams, A. J
• Sutherland, J. B

Titel

Identification of metabolites produced from N-phenylpiperazine by Mycobacterium spp

Ist Teil von

• Journal of industrial microbiology & biotechnology, 2007-03, Vol.34 (3), p.219-224

Ort / Verlag

Heidelberg: Berlin/Heidelberg : Springer-Verlag

Erscheinungsjahr

2007

Quelle

SpringerLink

Beschreibungen/Notizen

• Mycobacterium sp. 7E1B1W and seven other mycobacterial strains known to degrade hydrocarbons were investigated to determine their ability to metabolize the piperazine ring, a substructure found in many drugs. Cultures were grown at 30°C in tryptic soy broth and dosed with 3.1 mM N-phenylpiperazine hydrochloride; samples were removed at intervals and extracted with ethyl acetate. Two metabolites were purified from each of the extracts by high-performance liquid chromatography; they were identified by mass spectrometry and ¹H nuclear magnetic resonance spectroscopy as N-(2-anilinoethyl)acetamide and N-acetyl-N'-phenylpiperazine. The results show that mycobacteria have the ability to acetylate piperazine rings and cleave carbon-nitrogen bonds.