Details

Autor(en) / Beteiligte

• Baronti, Cécile
• Sire, Joséphine
• de Lamballerie, Xavier
• Quérat, Gilles

Titel

Nonstructural NS1 proteins of several mosquito-borne Flavivirus do not inhibit TLR3 signaling

Ist Teil von

• Virology (New York, N.Y.), 2010-09, Vol.404 (2), p.319-330

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2010

Quelle

ScienceDirect

Beschreibungen/Notizen

• Abstract Flaviviruses are single-stranded positive RNA viruses that replicate through double stranded RNA (dsRNA) intermediates. These dsRNA may be recognized as pathogen-associated molecular patterns by cellular receptors including membrane-bound Toll-like receptor 3 (TLR3) and cytosolic helicases RIG-I and MDA5. dsRNA stimulation results in signaling cascades converging to activation of interferon (IFN) regulatory factor 3 (IRF3) and to transcriptional activation of several interferon stimulated genes, including IFNß and inflammatory cytokines. There are conflicting reports concerning the ability of West Nile virus to counteract TLR3 signaling. In our analyses, transiently or stably expressed NS1 proteins from two West Nile viruses, two dengue 2 viruses and a yellow fever virus failed to inhibit TLR3 signaling in two different mammalian cell lines. Moreover, using siRNA inhibiting the helicase signalization pathway, we show that viral infection did not impede TLR3 responses to poly(I:C). We conclude that NS1 proteins from distinct mosquito-borne flaviviruses do not inhibit TLR3 signaling.