Molecular microbiology, 2010-07, Vol.77 (1), p.143-157
2010
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- Autor(en) / Beteiligte
- Titel
- Hyperosmosis enhances radiation and hydroxyurea resistance of Schizosaccharomyces pombe checkpoint mutants through the spindle checkpoint and delayed cytokinesis
- Ist Teil von
- Molecular microbiology, 2010-07, Vol.77 (1), p.143-157
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Summary The DNA damage and stress response pathways interact to regulate cellular responses to genotoxins and environmental stresses. How these pathways interact in Schizosaccharomyces pombe is not well understood. We demonstrate that osmotic stress suppresses the DNA damage sensitivity of checkpoint mutants, and that this occurs through three distinct cell cycle delays. A delay in G2/M is dependent on Srk1. Progression through mitosis is halted by the Mad2‐dependent spindle checkpoint. Finally, cytokinesis is impaired by modulating Cdc25 expression. These three delays, imposed by osmotic stress, together compensate for the loss of checkpoint signalling.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0950-382XeISSN: 1365-2958DOI: 10.1111/j.1365-2958.2010.07193.xTitel-ID: cdi_proquest_miscellaneous_754559474
- Format
- –
- Schlagworte
- Biological and medical sciences, cdc25 Phosphatases, Cell Cycle Proteins - metabolism, Cell division, Cytokines, Cytokinesis - drug effects, Cytokinesis - radiation effects, DNA damage, Drug resistance, Fundamental and applied biological sciences. Psychology, Gene expression, Hydroxyurea - toxicity, Mad2 Proteins, Microbiology, Miscellaneous, Mitogen-Activated Protein Kinases, Mutation, Mycology, Nuclear Proteins, Osmotic Pressure, Radiation, Schizosaccharomyces - drug effects, Schizosaccharomyces - growth & development, Schizosaccharomyces - physiology, Schizosaccharomyces - radiation effects, Schizosaccharomyces pombe, Schizosaccharomyces pombe Proteins - metabolism, Ultraviolet Rays, Yeast