Details

Autor(en) / Beteiligte

• Jin, Y.-C
• Wei, P
• Wei, X.-X
• Zhao, Z.-Y
• Li, Y

Titel

Rapid Detection of BF Haplotypes by a Semi-nested Polymerase Chain Reaction, which Causes Resistance/Susceptibility to Marek's Disease in Chicken

Ist Teil von

• Scandinavian journal of immunology, 2010-08, Vol.72 (2), p.94-97

Ort / Verlag

Oxford, UK: Oxford, UK : Blackwell Publishing Ltd

Erscheinungsjahr

2010

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• A semi-nested polymerase chain reaction (snPCR) assay was developed for the rapid detection of resistant/susceptible BF haplotypes to Marek's disease (MD) using the cDNA samples from peripheral blood leucocytes, liver, spleen and heart from Xiayan homozygous chickens: A₁₁, C₂₃, D₈ and D₁₂ (resistant to MD), A₅ and B₂₁ (susceptible to MD). The snPCR was utilized to span alternative splicing-out of the sequence encoding the second segment of the cytoplasmic part of the mature BF molecules (exon 7). This alternative exon 7 splice variant was detected in BF*A₅ and BF*B₂₁ (susceptible to MD), but not in the MD-resistant BF*A₁₁, BF*C₂₃, BF*D₈ and BF*D₁₂ haplotypes, suggesting a potential role of exon 7 for the detection of resistant/susceptible BF haplotypes to MD.