Details

Autor(en) / Beteiligte

• Lang, Gerhard
• Mayhudin, Nor Ainy
• Mitova, Maya I
• Sun, Lin
• van der Sar, Sonia
• Blunt, John W
• Cole, Anthony L. J
• Ellis, Gill
• Laatsch, Hartmut
• Munro, Murray H. G

Titel

Evolving Trends in the Dereplication of Natural Product Extracts: New Methodology for Rapid, Small-Scale Investigation of Natural Product Extracts

Ist Teil von

• Journal of natural products (Washington, D.C.), 2008-09, Vol.71 (9), p.1595-1599

Ort / Verlag

Washington, DC: American Chemical Society and American Society of Pharmacognosy

Erscheinungsjahr

2008

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The use of an HPLC bioactivity profiling/microtiter plate technique in conjunction with capillary probe NMR instrumentation and access to appropriate databases effectively short-circuits conventional dereplication procedures, necessarily based on multimilligram extracts, to a single, more rapid submilligram operation. This approach to dereplication is illustrated using fungal or bacterial extracts that contain known compounds. In each case the dereplication steps were carried out on microgram quantities of extract and demonstrate the discriminating power of 1H NMR spectroscopy as a definitive dereplication tool.