Details

Autor(en) / Beteiligte

• Stewart, Margaret L.
• Crouch, Robert J.
• Maizel, J.V.

Titel

A high-resolution oligonucleotide map generated by restriction of poliovirus type I genomic RNA by ribonuclease III

Ist Teil von

• Virology (New York, N.Y.), 1980-01, Vol.104 (2), p.375-397

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1980

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Processing of poliovirus Type I (Mahoney) genomic RNA with Escherichia coli ribonuclease III produces a large number of discrete fragments. Analysis of these fragments (and isolated oligonucleotides) using a high-resolution sodium dodecyl sulfate-agarose-acrylamide preparative gel and a two-dimensional fingerprinting gel system has allowed us to map 76 T 1 ribonuclease-resistant oligonucleotides within the poliovirus genome at 200- to 300-base intervals from the poly(A) end. Strikingly, the most preferred cleavage sites on the poliovirus genomic RNA are located close to each terminus, approximately 75 bases from the 5′ end, and 616 bases from the 3′ end. Furthermore, these and other internal sites appear to punctuate the translation products of the poliovirus mRNA intercistronically. Thus cleavage of poliovirus genomic RNA by ribonuclease III greatly resembles processing of bacteriophage T 7 early mRNA by this enzyme.