Details

Autor(en) / Beteiligte

• Li, Buhong, PhD
• Chen, Zheng
• Liu, Lina
• Huang, Zufang
• Huang, Zheng
• Xie, Shusen

Titel

Differences in sensitivity to HMME-mediated photodynamic therapy between EBV+ C666-1 and EBV− CNE2 cells

Ist Teil von

• Photodiagnosis and photodynamic therapy, 2010-09, Vol.7 (3), p.204-209

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• Summary Objectives The aim of this study was to compare the sensitivity of hematoporphyrin monomethyl ether (HMME)-mediated PDT in the Epstein-Barr virus (EBV) positive and negative human nasopharyngeal carcinoma (NPC) cells. Methods and materials The intracellular uptake of HMME in C666-1 (EBV+) and CNE2 (EBV−) cells was quantitatively determined after incubating with different concentration of HMME. The subcellular localization of HMME was examined under a laser scanning fluorescence microscopy. The survival rate of C666-1 and CNE2 cells after HMME PDT was evaluated with clonogenic assay. Results The EBV− CNE2 cells showed higher intracellular uptake of HMME and lower survival rate than EBV+ C666-1 cells. There was no significant difference in terms of subcellular localization of HMME between C666-1 and CNE2 cells. Conclusion The sensitivity of EBV− CNE2 cells to HMME-mediated PDT was significantly higher than that of EBV+ C666-1 cells. The intracellular uptake of HMME plays a key role in determining the PDT sensitivity.