Nano letters, 2010-07, Vol.10 (7), p.2493-2497
2010
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Details
- Autor(en) / Beteiligte
- Titel
- Detecting DNA Folding with Nanocapillaries
- Ist Teil von
- Nano letters, 2010-07, Vol.10 (7), p.2493-2497
- Ort / Verlag
- Washington, DC: American Chemical Society
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We demonstrate for the first time the detection of the folding state of double-stranded DNA in nanocapillaries with the resistive pulse technique. We show that glass capillaries can be pulled into nanocapillaries with diameters down to 45 nm. We study translocation of λ -DNA which is driven by an electrophoretic force through the nanocapillary. The resulting change in ionic current indicates the folding state of single λ -DNA molecules. Our experiments prove that nanocapillaries are suitable for label-free analysis of DNA in aqueous solutions and viable alternatives to solid-state nanopores made by silicon nanotechnology.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1530-6984eISSN: 1530-6992DOI: 10.1021/nl100997sTitel-ID: cdi_proquest_miscellaneous_748983881
- Format
- –
- Schlagworte
- Condensed matter: structure, mechanical and thermal properties, DNA - chemistry, Electrophoresis - instrumentation, Electrophoresis - methods, Equipment Design, Exact sciences and technology, Glass - chemistry, Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties, Nanostructures - chemistry, Nanotechnology - instrumentation, Nanotechnology - methods, Nucleic Acid Conformation, Physics, Surfaces and interfaces, thin films and whiskers (structure and nonelectronic properties)