Details

Autor(en) / Beteiligte

• Esona, Mathew Dioh
• Steele, Duncan
• Kerin, Tara
• Armah, George
• Peenze, Ina
• Geyer, Annelise
• Page, Nicola
• Nyangao, James
• Agbaya, Veronique Akran
• Trabelsi, Abdelhalim
• Tsion, Bizuneh
• Aminu, Maryam
• Sebunya, Theresia
• Dewar, John
• Glass, Roger
• Gentsch, Jon

Titel

Determination of the G and P Types of Previously Nontypeable Rotavirus Strains from the African Rotavirus Network, 1996–2004: Identification of Unusual G Types

Ist Teil von

• The Journal of infectious diseases, 2010-09, Vol.202 (Supplement-1), p.S49-S54

Ort / Verlag

United States: The University of Chicago Press

Erscheinungsjahr

2010

Link zum Volltext

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• A total of 215 nontypeable rotavirus samples collected from children <5 years of age by members of the African Rotavirus Network were characterized using reverse-transcription polymerase chain reaction analysis and sequencing. The most predominant strain identified was P[8]G1 (46.9%). Genotypes P[8]G10, P[8]G8, P[6]G8, and P[7]G5 were also detected at frequencies varying from 0.5% to 2.3%. This study suggests that reassortment of unusual G types into a background of globally common genotype P[8] strains may be a major mechanism of generating rotavirus diversity. Nucleotide substitutions at the P[8], P[6], and G1 primer binding sites accounted for the failure to type these strains initially. Hence, these findings highlight the need for regular evaluation of rotavirus genotyping methods.