American journal of physiology. Renal physiology, 2010-08, Vol.299 (2), p.F453-F464
2010
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Details
- Autor(en) / Beteiligte
- Titel
- Intravenous cell therapy for acute renal failure with serum amyloid A protein-reprogrammed cells
- Ist Teil von
- American journal of physiology. Renal physiology, 2010-08, Vol.299 (2), p.F453-F464
- Ort / Verlag
- United States: American Physiological Society
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Serum amyloid A protein (SAA), a prominent component of the acute-phase response, is strongly expressed in developing and repairing kidneys and promotes tubulogenesis. Accordingly, we reprogrammed relatively undifferentiated NRK52E cells with the mouse SAA1.1 gene and transplanted SAA-positive and -negative cells into rats with acute renal failure. We found that SAA-positive cells accelerated renal recovery in three models of acute renal failure: gentamicin nephrotoxicity, cisplatin-mediated renal injury, and ischemia-reperfusion renal injury. The dramatic improvement of renal failure was demonstrable within 2 days, consistent with an early paracrine effect. However, abundant donor cells were also found integrated in the healing tubular architecture after 7 days. We conclude that infusions of SAA-positive cells promote renal recovery after acute renal failure and offer a potentially powerful and novel therapy of renal failure.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1931-857XeISSN: 1522-1466DOI: 10.1152/ajprenal.00050.2010Titel-ID: cdi_proquest_miscellaneous_748938947
- Format
- –
- Schlagworte
- Acute Kidney Injury - genetics, Acute Kidney Injury - metabolism, Acute Kidney Injury - physiopathology, Acute Kidney Injury - therapy, Animals, Apoptosis, Cell Line, Cell Proliferation, Cell Transplantation, Cells, Cisplatin, Disease Models, Animal, Genes, Genetic Therapy - methods, Genotype, Gentamicins, Kidney - metabolism, Kidney - pathology, Kidney - physiopathology, Kidney diseases, Kidneys, Mice, Nephrology, Organic Anion Transport Protein 1 - metabolism, Phenotype, Proliferating Cell Nuclear Antigen - metabolism, Proteins, Rats, Rats, Sprague-Dawley, Recovery of Function, Reperfusion Injury - genetics, Reperfusion Injury - metabolism, Reperfusion Injury - physiopathology, Reperfusion Injury - therapy, Serum Amyloid A Protein - biosynthesis, Serum Amyloid A Protein - genetics, Sodium-Hydrogen Exchanger 3, Sodium-Hydrogen Exchangers - metabolism, Time Factors, Transfection