Details

Autor(en) / Beteiligte

• Boyle, Michael D.P.
• Langone, John J.
• Borsos, Tibor

Titel

1,10-Phenanthroline mediated hemolysis—evidence that 1,10-phenanthroline does not substitute specifically for C9

Ist Teil von

• Molecular immunology, 1979-05, Vol.16 (5), p.307-312

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

1979

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• We have re-examined the interaction of 1,10 phenanthroline with E, EAC1–8 and various other red cell intermediates. Two major findings emerged from this study: (1) 1,10-phenanthroline could mediate lysis of E, EAC4, EAC1–7 or EAC1–8. Under identical conditions of dose, pH and temperature, the rate (though not the extent) of lysis varied for different red cell intermediates, with EAC1–8 being lysed the most rapidly, followed by EAC1–7, EAC4 and E. (2) An intermediate could be isolated following the interaction of E and 1,10-phenanthroline that would lyse in the absence of fluid phase factors. This intermediate could be prevented from releasing its hemoglobin by addition of high molarity EDTA or EGTA. This cell was functionally similar to the E* state isolated during the lysis of E by anti-Forssman antibody and GPC.