Details

Autor(en) / Beteiligte

• Derkx, F H
• Tan-Tjiong, H L
• Man in't Veld, A J
• Schalekamp, M P
• Schalekamp, M A

Titel

Activation of inactive plasma renin by tissue kallikreins

Ist Teil von

• The journal of clinical endocrinology and metabolism, 1979-11, Vol.49 (5), p.765-769

Ort / Verlag

United States

Erscheinungsjahr

1979

Quelle

MEDLINE

Beschreibungen/Notizen

• It has been reported that inactive (acid-activable) human renin could be converted into the active form by adding urinary kallikrein to acid-pretreated plasma. Without prior acidification, however, only a small portion of the total amount of inactive renin present in plasma was converted (activated) by kallikrein, probably because native plasma contains protease (kallikrein) inhibitors that are destroyed by acid. We have separated inactive and active renin by DEAE-Sepharose column chromatography of normal human plasma at pH 7.5 and a linearly increasing sodium gradient. Inactive renin isolated in this way could be activated at pH 7.5 by highly purified pancreas and urinary kallikreins. With the semipurified preparation of inactive renin, prior acidification was not required for obtaining virtually complete activation by kallikrein. The kallikreins were effective at concentrations as low as 1 x 10(-8) mol/liter. It is therefore possible that one or more tissue kallikreins act as physiological activators of inactive renin.