Mutagenesis, 2010-03, Vol.25 (2), p.149-154
2010
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- Autor(en) / Beteiligte
- Titel
- Genotoxic effects of neutrophils and hypochlorous acid
- Ist Teil von
- Mutagenesis, 2010-03, Vol.25 (2), p.149-154
- Ort / Verlag
- Oxford: Oxford University Press
- Erscheinungsjahr
- 2010
- Quelle
- Oxford Journals 2020 Medicine
- Beschreibungen/Notizen
- Chronic inflammation has been recognized as a contributing factor in the pathogenesis of lung cancer. In this process, reactive oxygen species released by neutrophils may play an important role. The aim of the present study was to investigate the capacity of the major neutrophilic oxidant hypochlorous acid (HOCl), which is formed by myeloperoxidase (MPO), to induce DNA damage and mutagenicity in lung cells. HOCl was mutagenic in lung epithelial A549 cells in vitro, showing at physiological concentrations a significant induction of mutations in the HPRT gene. We studied three major types of DNA lesions that could be relevant for this HOCl-induced mutagenicity. Single strand DNA breakage and 8-oxo-7,8-dihydro-2′-deoxyguanosine were not found to be increased following HOCl treatment. On the other hand, HOCl caused a significant increase in the formation of 3-(2-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-α]purin-10(3H)-one (M1dG), which can be formed by either malondialdehyde (MDA) or base propenals. We observed an increased MDA formation upon exposure of A549 cells to HOCl, but a role of base propenals cannot be excluded. In line with this, we observed 4-fold increased M1dG adduct levels in mice that were intratracheally instilled with lipopolysaccharide to induce a pulmonary inflammation with neutrophil influx. Depletion of circulating neutrophils significantly reduced pulmonary MPO activity as well as M1dG adducts levels, thereby providing a causal link between neutrophils/HOCl and pulmonary genotoxicity in vivo. Taken together, these data indicate that MPO catalysed formation of HOCl during lung inflammation should be considered as a significant source of neutrophil-induced genotoxicity.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0267-8357eISSN: 1464-3804DOI: 10.1093/mutage/gep053Titel-ID: cdi_proquest_miscellaneous_746233202
- Format
- –
- Schlagworte
- Adducts, Adenoma - drug therapy, Adenoma - metabolism, Adenoma - pathology, Animals, Biological and medical sciences, Cells, Cultured, Colony-Forming Units Assay, Data processing, Deoxyguanosine - analogs & derivatives, Deoxyguanosine - metabolism, DNA Adducts, DNA Breaks, Single-Stranded - drug effects, DNA damage, DNA Damage - drug effects, Fundamental and applied biological sciences. Psychology, Genotoxicity, Humans, Hypochlorous acid, Hypochlorous Acid - toxicity, Hypoxanthine phosphoribosyltransferase, Hypoxanthine Phosphoribosyltransferase - genetics, Hypoxanthine Phosphoribosyltransferase - metabolism, Inflammation, Inflammation - chemically induced, Leukocytes (neutrophilic), Lipid Peroxidation - drug effects, Lipopolysaccharides, Lipopolysaccharides - pharmacology, Lung - drug effects, Lung - metabolism, Lung cancer, Lung Neoplasms - drug therapy, Lung Neoplasms - metabolism, Lung Neoplasms - pathology, Male, Malondialdehyde, Malondialdehyde - metabolism, Mice, Mice, Inbred C57BL, Molecular and cellular biology, Molecular genetics, Mutagenesis, Mutagenesis. Repair, Mutagenicity, Mutation, Mutation - genetics, Neutrophils - metabolism, Oxidants, Oxidants - toxicity, Peroxidase, Peroxidase - metabolism, Purine Nucleosides - metabolism, Reactive oxygen species