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MR Imaging of Inflammation during Myelin-Specific T Cell-Mediated Autoimmune Attack in the EAE Mouse Spinal Cord
Ist Teil von
Molecular imaging and biology, 2010-06, Vol.12 (3), p.240-249
Ort / Verlag
New York: Springer-Verlag
Erscheinungsjahr
2010
Quelle
MEDLINE
Beschreibungen/Notizen
Purpose
The purpose of this study is to detect myelin-specific T cells, key pathological mediators in early multiple sclerosis, and the corresponding animal model, experimental autoimmune encephalomyelitis (EAE), in the mouse spinal cord.
Procedures
T cells were labeled with the iron-based, magnetic resonance (MR) contrast reagent, Feridex, and the transfection reagent, protamine sulfate, resulting in ∼100% iron-labeling efficiency. Feridex-labeling did not alter the induction of EAE by T cells, and recipients were imaged by a 12-T MR instrument.
Results
Focal hypointense lesions were resolvable to gray or white matter of the lumbar spinal cord in T
2
-weighted images of the recipients of Feridex-labeled T cells. Lesions corresponded to histological evidence of inflammatory lesions and iron-labeled cells in eight-of-eight mice. In contrast, hypointense lesions were not observed eight-of-eight recipients of unlabeled T cells.
Conclusions
These results demonstrate and provide methodologies for labeling, detecting, and extracting MRI-detectable foci of iron-labeled cells.