Details

Autor(en) / Beteiligte

• Robinson, Kristine M.
• Njus, Jeffrey M.
• Phillips, Daniel A.
• Proctor, Thomas M.
• Rooney, William D.
• Jones, Richard E.

Titel

MR Imaging of Inflammation during Myelin-Specific T Cell-Mediated Autoimmune Attack in the EAE Mouse Spinal Cord

Ist Teil von

• Molecular imaging and biology, 2010-06, Vol.12 (3), p.240-249

Ort / Verlag

New York: Springer-Verlag

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• Purpose The purpose of this study is to detect myelin-specific T cells, key pathological mediators in early multiple sclerosis, and the corresponding animal model, experimental autoimmune encephalomyelitis (EAE), in the mouse spinal cord. Procedures T cells were labeled with the iron-based, magnetic resonance (MR) contrast reagent, Feridex, and the transfection reagent, protamine sulfate, resulting in ∼100% iron-labeling efficiency. Feridex-labeling did not alter the induction of EAE by T cells, and recipients were imaged by a 12-T MR instrument. Results Focal hypointense lesions were resolvable to gray or white matter of the lumbar spinal cord in T 2 -weighted images of the recipients of Feridex-labeled T cells. Lesions corresponded to histological evidence of inflammatory lesions and iron-labeled cells in eight-of-eight mice. In contrast, hypointense lesions were not observed eight-of-eight recipients of unlabeled T cells. Conclusions These results demonstrate and provide methodologies for labeling, detecting, and extracting MRI-detectable foci of iron-labeled cells.