Details

Autor(en) / Beteiligte

• Barros, Silvana P.
• Wirojchanasak, Sodsi
• Barrow, David A.
• Panagakos, Fotinos S.
• DeVizio, William
• Offenbacher, Steven

Titel

Triclosan inhibition of acute and chronic inflammatory gene pathways

Ist Teil von

• Journal of clinical periodontology, 2010-05, Vol.37 (5), p.412-418

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

2010

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Barros SP, Wirojchanasak S, Barrow DA, Panagakos F, Devizio W, Offenbacher S. Triclosan inhibition of acute and chronic inflammatory gene pathways. J Clin Periodontol 2010; 37: 412–418. doi: 10.1111/j.1600‐051X.2010.01548.x. Aim: We sought to determine whether triclosan (2,4,4′‐trichloro‐2′‐hydroxydiphenylether), an extensively used anti‐plaque agent with broad‐spectrum anti‐microbial activity, with reported anti‐inflammatory effects via inhibition of prostaglandin E2 and interleukin 1 (IL‐1)β, could also more broadly suppress multiple inflammatory gene pathways responsible for the pathogenesis of gingivitis and periodontitis. Materials and Methods: As an exploratory study, the effects of triclosan on the inflammatory gene expression profile were assessed ex vivo using peripheral whole blood samples from eight periodontally healthy donors. Ten‐millilitres whole blood aliquots were incubated 2 h with 0.3 μg/ml Escherichia coli lipopolysaccharide (LPS) with or without 0.5 μg/ml triclosan. Affymetrix microarray gene expression profiles from isolated leucocytes and pathway‐specific quantitative polymerase chain reaction arrays were used to investigate changes in expression of target cytokines and cell signalling molecules. Results: Ex vivo human whole blood assays indicated that triclosan significantly down‐regulated the LPS‐stimulated expression of Toll‐like receptor signalling molecules and other multiple inflammatory molecules including IL‐1 and IL‐6 and the dampening of signals that activate the T‐helper type 1 acquired immune response via suppression of CD70 with concomitant up‐regulation of growth factors related to bone morphogenetic protein (BMP)2 and BMP6 synthesis. Conclusions: Anti‐inflammatory effects were found in this exploratory survey, including suppression of microbial‐pathogen recognition pathway molecules and the suppression of acute and chronic mediators of inflammation.