Details

Autor(en) / Beteiligte

• Adhikari, Bal Ram
• Pandey, Basu Dev
• Ghimire, Prakash
• Shrestha, Bhawana
• Khadka, Manoj
• Yoda, Tomoko
• Suzuki, Yasuhiko

Titel

Loop-Mediated Isothermal Amplification (LAMP) for the Direct Detection of Human Pulmonary Infections with Environmental (Nontuberculosis) Mycobacteria

Ist Teil von

• Japanese Journal of Infectious Diseases, 2009/05/29, Vol.62(3), pp.212-214

Ort / Verlag

Japan: National Institute of Infectious Diseases

Erscheinungsjahr

2009

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Most first-line anti-tuberculosis drugs have less in vitro activity against atypical mycobacteria. Loop-mediated isothermal amplification (LAMP) was used for the rapid diagnosis of mycobacterial species. The sensitivity of LAMP was 96.1% (49/51) in smear-positive and culture-positive sputum samples and 85.0% (17/20) in smear-negative and culture-positive samples. Of the 77 total LAMP-positive samples, 75 (97.4%) were identified as Mycobacterium tuberculosis and 2 (2.6%) as M. intracellulare. One of the M. intracellulare-infected cases was identified in a patient with suspected mycobacteriosis and another was found in a follow-up patient.