Details

Autor(en) / Beteiligte

• Jamal, P.
• Tompang, M. Fahrurrazi
• Alam, M. Zahangir

Titel

Optimization of Media Composition for the Production of Bioprotein from Pineapple Skins by Liquid-State Bioconversion

Ist Teil von

• Journal of applied sciences (Asian Network for Scientific Information), 2009-08, Vol.9 (17), p.3104-3109

Erscheinungsjahr

2009

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Two-steps optimization strategy based on statistical experimental design was carried out to enhance bioprotein production by liquid state bioconversion utilizing pineapple skins as substrate. A two-level Plackett-Burman design was employed first where 11 variables were studied for their influence on bioprotein production. Pineapple Skins Soluble (PSS) sugar content, KH sub(2)PO sub(4) and NH sub(4)H sub(2)PO sub(4) were the most significant variables for improving bioprotein production. A three-level Central Composite Design (CCD) was employed for maximizing the bioprotein production. A mathematical model was developed to show the effects of each medium component and their combinatorial interaction on bioprotein production. The optimal medium composition for maximum bioprotein production was PSS content 1.0%, KH sub(2)PO sub(4) 0.1% and NH sub(4)H sub(2)PO sub(4) 0.25%. The final experiment was carried out to validate the model, which has shown to produce 514.2 g kg super(-1) bioprotein on the 5th day of fermentation with a slight increase of 0.02% from the experimental run in CCD.