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Reversibly immortalized human olfactory ensheathing glia from an elderly donor maintain neuroregenerative capacity
Glia, 2010-04, Vol.58 (5), p.546-558
Lim, Filip
Martín-Bermejo, M. Jesús
García-Escudero, Vega
Gallego-Hernández, M. Teresa
García-Gómez, Ana
Rábano, Alberto
Díaz-Nido, Javier
Ávila, Jesús
Moreno-Flores, M. Teresa
2010
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Lim, Filip
Martín-Bermejo, M. Jesús
García-Escudero, Vega
Gallego-Hernández, M. Teresa
García-Gómez, Ana
Rábano, Alberto
Díaz-Nido, Javier
Ávila, Jesús
Moreno-Flores, M. Teresa
Titel
Reversibly immortalized human olfactory ensheathing glia from an elderly donor maintain neuroregenerative capacity
Ist Teil von
Glia, 2010-04, Vol.58 (5), p.546-558
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2010
Quelle
Wiley-Blackwell Journals
Beschreibungen/Notizen
A continuous normal function of olfactory ensheathing glia (OEG) is to promote axonal regeneration from the olfactory neuroepithelium to the brain, and their neuroregenerative potential in other CNS sites such as the injured spinal cord has been studied for over a decade. However, human OEG are difficult to obtain in large amounts directly from tissues, and the derived primary cultures have a limited duplication capacity. Thus, although auto‐transplantation may be an obvious option for initial proof‐of‐concept trials, alternatives must be explored to obtain large quantities of homogeneous, pre‐characterized OEG for wide‐scale therapeutic use. We have cultured primary human OEG derived from olfactory bulbs (OB) obtained by necropsy and successfully extended the replicative lifespan of these cells using lentivectors encoding Bmi‐1 and TERT transgenes flanked by loxP sites. In contrast to the primary cells which could only be expanded for a limited number of passages (∼12), adult human OEG immortalized Bmi‐1/TERT divided indefinitely in culture. Clonal lines were isolated and the floxed transgenes could be excised by lentivector‐mediated Cre recombinase delivery. Primary, immortalized, and deimmortalized human OEG all expressed typical markers of this cell type and importantly, were all able to promote axonal regeneration of adult rat retinal ganglion neurons (RGN) in co‐culture assays. © 2009 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0894-1491
eISSN: 1098-1136
DOI: 10.1002/glia.20944
Titel-ID: cdi_proquest_miscellaneous_744715981
Format
–
Schlagworte
Adolescent
,
Adult
,
adult human olfactory ensheathing glia
,
Animals
,
Animals, Newborn
,
axonal regeneration
,
Cells, Cultured
,
central nervous system repair
,
Clone Cells
,
Coculture Techniques - methods
,
Female
,
Green Fluorescent Proteins - genetics
,
Humans
,
lentivector transduction
,
Male
,
Microtubule-Associated Proteins - metabolism
,
Middle Aged
,
Nerve Regeneration - physiology
,
Nerve Tissue Proteins - genetics
,
Nerve Tissue Proteins - metabolism
,
Neuroglia - physiology
,
Neuroglia - transplantation
,
Nuclear Proteins - genetics
,
Nuclear Proteins - metabolism
,
Olfactory Bulb - cytology
,
Polycomb Repressive Complex 1
,
Proto-Oncogene Proteins - genetics
,
Proto-Oncogene Proteins - metabolism
,
Rats
,
Repressor Proteins - genetics
,
Repressor Proteins - metabolism
,
Retinal Ganglion Cells - metabolism
,
retinal ganglion neurons
,
reversibly immortalized cell lines
,
Spinal Cord Injuries - surgery
,
Telomerase - genetics
,
Telomerase - metabolism
,
Transduction, Genetic - methods
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