The Plant journal : for cell and molecular biology, 2010-04, Vol.62 (1), p.52-63
2010
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- Autor(en) / Beteiligte
- Titel
- Skp1-like protein SSK1 is required for cross-pollen compatibility in S-RNase-based self-incompatibility
- Ist Teil von
- The Plant journal : for cell and molecular biology, 2010-04, Vol.62 (1), p.52-63
- Ort / Verlag
- Oxford, UK: Oxford, UK : Blackwell Publishing Ltd
- Erscheinungsjahr
- 2010
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- The self-incompatibility (SI) response occurs widely in flowering plants as a means of preventing self-fertilization. In these self/non-self discrimination systems, plant pistils reject self or genetically related pollen. In the Solanaceae, Plantaginaceae and Rosaceae, pistil-secreted S-RNases enter the pollen tube and function as cytotoxins to specifically arrest self-pollen tube growth. Recent studies have revealed that the S-locus F-box (SLF) protein controls the pollen expression of SI in these families. However, the precise role of SLF remains largely unknown. Here we report that PhSSK1 (Petunia hybrida SLF-interacting Skp1-like1), an equivalent of AhSSK1 of Antirrhinum hispanicum, is expressed specifically in pollen and acts as an adaptor in an SCF(Skp1-Cullin1-F-box)SLF complex, indicating that this pollen-specific SSK1-SLF interaction occurs in both Petunia and Antirrhinum, two species from the Solanaceae and Plantaginaceae, respectively. Substantial reduction of PhSSK1 in pollen reduced cross-pollen compatibility (CPC) in the S-RNase-based SI response, suggesting that the pollen S determinant contributes to inhibiting rather than protecting the S-RNase activity, at least in solanaceous plants. Furthermore, our results provide an example that a specific Skp1-like protein other than the known conserved ones can be recruited into a canonical SCF complex as an adaptor.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0960-7412eISSN: 1365-313XDOI: 10.1111/j.1365-313X.2010.04123.xTitel-ID: cdi_proquest_miscellaneous_744671509
- Format
- –
- Schlagworte
- Amino Acid Sequence, Antirrhinum, Botany, cross-pollen compatibility, Flowers & plants, Gene Expression Regulation, Plant, Gene Knockdown Techniques, Genetics, Inbreeding, Molecular Sequence Data, Petunia, Petunia - genetics, Petunia hybrida, Plant Proteins - genetics, Plant Proteins - metabolism, Plantaginaceae, Pollen, Pollen - genetics, Proteins, Ribonucleases - genetics, Ribonucleases - metabolism, Rosaceae, S-locus F-box protein, S-RNase, self-incompatibility, Sequence Alignment, SKP Cullin F-Box Protein Ligases - genetics, SKP Cullin F-Box Protein Ligases - metabolism, Solanaceae, SSK1