Science (American Association for the Advancement of Science), 2004-03, Vol.303 (5663), p.1526-1529
2004
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Details
- Autor(en) / Beteiligte
- Titel
- Species-Specific Recognition of Single-Stranded RNA via Toll-like Receptor 7 and 8
- Ist Teil von
- Science (American Association for the Advancement of Science), 2004-03, Vol.303 (5663), p.1526-1529
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2004
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Double-stranded ribonucleic acid (dsRNA) serves as a danger signal associated with viral infection and leads to stimulation of innate immune cells. In contrast, the immunostimulatory potential of single-stranded RNA (ssRNA) is poorly understood and innate immune receptors for ssRNA are unknown. We report that guanosine (G)- and uridine (U)-rich ssRNA oligonucleotides derived from human immunodeficiency virus-1 (HIV-1) stimulate dendritic cells (DC) and macrophages to secrete interferon-α and proinflammatory, as well as regulatory, cytokines. By using Toll-like receptor (TLR)-deficient mice and genetic complementation, we show that murine TLR7 and human TLR8 mediate species-specific recognition of GU-rich ssRNA. These data suggest that ssRNA represents a physiological ligand for TLR7 and TLR8.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1093620Titel-ID: cdi_proquest_miscellaneous_743461527
- Format
- –
- Schlagworte
- Adaptor Proteins, Signal Transducing, Animals, Antigens, Differentiation - metabolism, B lymphocytes, Base Sequence, Biological and medical sciences, Cellular immunity, Chemical properties, Cytokines, Cytokines - biosynthesis, Dendritic Cells - immunology, Fatty Acids, Monounsaturated, Fundamental and applied biological sciences. Psychology, Genetic Complementation Test, Genetics, Guanosine - analysis, HIV, HIV 1, HIV antibodies, HIV-1 - genetics, HIV-1 - immunology, Human immunodeficiency virus, Human immunodeficiency virus 1, Humans, Immune system, Individualized Instruction, Infections, Interferon-alpha - biosynthesis, Leukocytes, Mononuclear - immunology, Ligands, Macrophages - immunology, Membrane Glycoproteins - genetics, Membrane Glycoproteins - metabolism, Mice, Mice, Inbred C57BL, Microbiology, Myeloid cells, Myeloid Differentiation Factor 88, Oligoribonucleotides - chemistry, Oligoribonucleotides - immunology, Pattern Recognition, Quaternary Ammonium Compounds, Receptors, Cell Surface - genetics, Receptors, Cell Surface - metabolism, Receptors, Immunologic - metabolism, Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains, Ribonucleic acid, RNA, RNA, Viral - chemistry, RNA, Viral - immunology, RNA, Viral - metabolism, Secretion, Species Specificity, Thionucleotides - chemistry, Thionucleotides - immunology, TLR7 protein, TLR8 protein, Toll-Like Receptor 7, Toll-Like Receptor 8, Toll-Like Receptors, Transfection, Uridine - analysis, Virology