Nature, 2004-11, Vol.432 (7015), p.353-360
2004
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- Autor(en) / Beteiligte
- Titel
- Regulation of p53 activity through lysine methylation
- Ist Teil von
- Nature, 2004-11, Vol.432 (7015), p.353-360
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2004
- Quelle
- EBSCOhost Psychology and Behavioral Sciences Collection
- Beschreibungen/Notizen
- p53 is a tumour suppressor that regulates the cellular response to genotoxic stresses. p53 is a short-lived protein and its activity is regulated mostly by stabilization via different post-translational modifications. Here we report a novel mechanism of p53 regulation through lysine methylation by Set9 methyltransferase. Set9 specifically methylates p53 at one residue within the carboxyl-terminus regulatory region. Methylated p53 is restricted to the nucleus and the modification positively affects its stability. Set9 regulates the expression of p53 target genes in a manner dependent on the p53-methylation site. The crystal structure of a ternary complex of Set9 with a p53 peptide and the cofactor product S-adenosyl-l-homocysteine (AdoHcy) provides the molecular basis for recognition of p53 by this lysine methyltransferase.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0028-0836eISSN: 1476-4687DOI: 10.1038/nature03117Titel-ID: cdi_proquest_miscellaneous_743416050
- Format
- –
- Schlagworte
- Amino Acid Sequence, Apoptosis, Cell Line, Cell Nucleus - metabolism, Gene expression, Gene Expression Regulation, Genes, p53 - genetics, Genes, ras - genetics, Histone-Lysine N-Methyltransferase - chemistry, Histone-Lysine N-Methyltransferase - genetics, Histone-Lysine N-Methyltransferase - metabolism, Humans, Lysine - metabolism, Methylation, Models, Molecular, Molecular Sequence Data, Promoter Regions, Genetic - genetics, Protein Binding, Protein Conformation, Protein Methyltransferases, Proteins, RNA, Messenger - genetics, RNA, Messenger - metabolism, S-Adenosylhomocysteine - metabolism, Substrate Specificity, Thermodynamics, Tumor Suppressor Protein p53 - chemistry, Tumor Suppressor Protein p53 - metabolism, Tumors