Science (American Association for the Advancement of Science), 2005-04, Vol.308 (5722), p.693-696
2005
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- Autor(en) / Beteiligte
- Titel
- PERIOD1-Associated Proteins Modulate the Negative Limb of the Mammalian Circadian Oscillator
- Ist Teil von
- Science (American Association for the Advancement of Science), 2005-04, Vol.308 (5722), p.693-696
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2005
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- The clock proteins PERIOD1 (PER1) and PERIOD2 (PER2) play essential roles in a negative transcriptional feedback loop that generates circadian rhythms in mammalian cells. We identified two PER1-associated factors, NONO and WDR5, that modulate PER activity. The reduction of NONO expression by RNA interference (RNAi) attenuated circadian rhythms in mammalian cells, and fruit flies carrying a hypomorphic allele were nearly arrhythmic. WDR5, a subunit of histone methyltransferase complexes, augmented PER-mediated transcriptional repression, and its reduction by RNAi diminished circadian histone methylations at the promoter of a clock gene.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1107373Titel-ID: cdi_proquest_miscellaneous_743385550
- Format
- –
- Schlagworte
- 3T3 Cells, Animals, Biological and medical sciences, Carrier Proteins - genetics, Carrier Proteins - metabolism, Cell Cycle Proteins, Cell Line, Cell lines, Cell nucleus, Chronobiology, Circadian Rhythm, Circadian rhythms, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, Drosophila - genetics, Drosophila - physiology, Drosophila Proteins - genetics, Drosophila Proteins - physiology, Feedback (Response), Female, Fractions, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation, Genes, Histones, Histones - metabolism, Immunoprecipitation, Individualized Instruction, Insects, Male, Mammals, Messenger RNA, Methylation, Mice, Mice, Inbred BALB C, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Nuclear Proteins - physiology, Nuclear Receptor Subfamily 1, Group D, Member 1, Period Circadian Proteins, Promoter Regions, Genetic, Proteins, Proteins - genetics, Proteins - metabolism, Rats, Receptors, Cytoplasmic and Nuclear - genetics, Receptors, Cytoplasmic and Nuclear - metabolism, Ribonucleic acid, RNA, RNA Interference, Transcription Factors, Transcription, Genetic, Transfection, Vertebrates: anatomy and physiology, studies on body, several organs or systems, Visual Acuity