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Indole-3-acetic acid biosynthesis in the mutant maize orange pericarp, a tryptophan auxotroph
Ist Teil von
Science (American Association for the Advancement of Science), 1991-11, Vol.254 (5034), p.998-1000
Ort / Verlag
United States: American Society for the Advancement of Science
Erscheinungsjahr
1991
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
The maize mutant orange pericarp is a tryptophan auxotroph, which results from mutation of two unlinked loci of tryptophan synthase B. This mutant was used to test the hypothesis that tryptophan is the precursor to the plant hormone indole-3-acetic acid (IAA). Total IAA in aseptically grown mutant seedlings was 50 times greater than in normal seedlings. In mutant seedlings grown on media containing stable isotope-labeled precursors, IAA was more enriched than was tryptophan. No incorporation of label into IAA from tryptophan could be detected. These results establish that IAA can be produced de novo without tryptophan as an intermediate