Science (American Association for the Advancement of Science), 2003-09, Vol.301 (5639), p.1531-1534
2003
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Glycosylation Restores Survival of Chilled Blood Platelets
- Ist Teil von
- Science (American Association for the Advancement of Science), 2003-09, Vol.301 (5639), p.1531-1534
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2003
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- Cooling of blood platelets clusters the von Willebrand factor receptor complex. Macrophage$\alpha_M\beta_2$integrins bind to the$GPIb\alpha$subunit of the clustered complex, resulting in rapid clearance of transfused, cooled platelets. This precludes refrigeration of platelets for transfusion, but the current practice of room temperature storage has major drawbacks. We document that$\alpha_M\beta_2$is a lectin that recognizes exposed$\beta-N-acetylglucosamine$residues of N-linked glycans on$GPIb\alpha$. Enzymatic galactosylation of chilled platelets blocks$\alpha_M\beta_2$recognition, prolonging the circulation of functional cooled platelets. Platelet-associated galactosyltransferase produces efficient galactosylation when uridine phosphate-galactose is added, affording a potentially simple method for storing platelets in the cold.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1085322Titel-ID: cdi_proquest_miscellaneous_743215495
- Format
- –
- Schlagworte
- Acetylglucosamine - metabolism, Acetylglucosamine - pharmacology, Animals, Bacteria, Biological and medical sciences, Blood, Blood platelets, Blood Platelets - metabolism, Blood Platelets - physiology, Blood Preservation, Blood transfusion, Blood transfusions, Carbohydrate Conformation, Cell Line, Cell Survival, Climate, Cold storage, Cold Temperature, Cooling, Diverse techniques, Erythrocytes, Female, Fundamental and applied biological sciences. Psychology, Galactose - metabolism, Galactosyltransferases - metabolism, Glycosylation, Humans, Integrins, Lectins, Lectins - metabolism, Ligands, Macrophage-1 Antigen - metabolism, Male, Mice, Mice, Inbred C57BL, Molecular and cellular biology, Monosaccharides - pharmacology, Phagocytosis, Phagocytosis - drug effects, Platelet Aggregation, Platelet Glycoprotein GPIb-IX Complex - metabolism, Platelet Membrane Glycoproteins, Platelet Transfusion, Platelets, Polysaccharides, Protection and preservation, Receptors, Refrigeration, Room temperature, Storage, Temperature, Uridine Diphosphate Galactose - metabolism, von Willebrand Factor - metabolism