Details

Autor(en) / Beteiligte

• Sönnichsen, B
• Koski, L. B
• Walsh, A
• Marschall, P
• Neumann, B
• Brehm, M
• Alleaume, A.-M
• Artelt, J
• Bettencourt, P
• Cassin, E
• Hewitson, M
• Holz, C
• Khan, M
• Lazik, S
• Martin, C
• Nitzsche, B
• Ruer, M
• Stamford, J
• Winzi, M
• Heinkel, R
• Röder, M
• Finell, J
• Häntsch, H
• Jones, S. J. M
• Jones, M
• Piano, F
• Gunsalus, K. C
• Oegema, K
• Gönczy, P
• Coulson, A
• Hyman, A. A
• Echeverri, C. J

Titel

Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans

Ist Teil von

• Nature, 2005-03, Vol.434 (7032), p.462-469

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2005

Quelle

Psychology & Behavioral Sciences Collection

Beschreibungen/Notizen

• A key challenge of functional genomics today is to generate well-annotated data sets that can be interpreted across different platforms and technologies. Large-scale functional genomics data often fail to connect to standard experimental approaches of gene characterization in individual laboratories. Furthermore, a lack of universal annotation standards for phenotypic data sets makes it difficult to compare different screening approaches. Here we address this problem in a screen designed to identify all genes required for the first two rounds of cell division in the Caenorhabditis elegans embryo. We used RNA-mediated interference to target 98% of all genes predicted in the C. elegans genome in combination with differential interference contrast time-lapse microscopy. Through systematic annotation of the resulting movies, we developed a phenotypic profiling system, which shows high correlation with cellular processes and biochemical pathways, thus enabling us to predict new functions for previously uncharacterized genes.