Peptides (New York, N.Y. : 1980), 1981-01, Vol.2 (4), p.445-452
1981
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Details
- Autor(en) / Beteiligte
- Titel
- Tritium labeling of gonadotropin releasing hormone in its proline and histidine residues
- Ist Teil von
- Peptides (New York, N.Y. : 1980), 1981-01, Vol.2 (4), p.445-452
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 1981
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- 3,4-dehydroproline 9-GnRH prepared by solid phase peptide synthesis was tritiated catalytically under various conditions yielding 3H-GnRH with specific radioactivities in the range from 35 to 60 Ci/mmol and full LH releasing activity in vitro. Using palladium/alumina catalyst, the tritiation of the double bond occurs within ten minutes. Investigation of the tritium distribution between the amino acid residues showed a remarkably high incorporation of tritium into the histidine residue (11 to 37%). On the basis of this observation, the tritium labeling of GnRH and angiotensin I by direct catalytic hydrogentritium exchange was found to be useful for the labeling of these peptides at remarkably high specific radioactivity.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0196-9781eISSN: 1873-5169DOI: 10.1016/S0196-9781(81)80103-9Titel-ID: cdi_proquest_miscellaneous_73901623
- Format
- –
- Schlagworte
- Aluminum Oxide, Angiotensin I, Animals, Catalysis, Catalytic tritiation, GnRH, Gonadotropin-Releasing Hormone - pharmacology, Half-Life, Histidine, Histidine labeling, Isotope Labeling - methods, Palladium, Pituitary Gland - drug effects, Proline - analogs & derivatives, Proline labeling, Rats, Tritium, Tritium labeling