Details

Autor(en) / Beteiligte

• Gonzalez, J.M. (Alcala de Henares Univ., Madrid (Spain). Faculty of Pharmacology, Dept. of Microbiology and Parasitology)
• Gonzalez, A.J
• Caabeiro, F.R

Titel

Purification of Trichinella spiralis tubulin: comparison of several analytic procedures

Ist Teil von

• Veterinary parasitology, 1998-06, Vol.77 (2), p.115-121

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

1998

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• This study compares the purity indices found after purifying tubulin obtained from the nematode parasite Trichinella spiralis, using different chromatographic and electrophoretic methods. Affinity chromatography, using monoclonal antibodies anti- α and anti- β-tubulin fixed to activated Sepharosa 4B-CNBr, yields a tubulin purity of 15%. In contrast, by means of interchange-anionic chromatography using a column of DEAE-Sephadex-A50, we obtained an increase in purity of up to 75%. Finally, with the combined application of preparative electrophoresis and electroelution of proteins in polyacrylamide gels with SDS, we obtained the best results with a purity reaching 90%.