Plant physiology (Bethesda), 1998-09, Vol.118 (1), p.125-135
1998
Details
- Autor(en) / Beteiligte
- Titel
- Purification and characterization of peroxidases correlated with lignification in poplar xylem
- Ist Teil von
- Plant physiology (Bethesda), 1998-09, Vol.118 (1), p.125-135
- Ort / Verlag
- Rockville, MD: American Society of Plant Physiologists
- Erscheinungsjahr
- 1998
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Lignin is an integral cell wall component of all vascular plants. Peroxidases are widely believed to catalyze the last enzymatic step in the biosynthesis of lignin, the dehydrogenation of the p-coumaryl alcohols. As the first stage in identifying lignin-specific peroxidase isoenzymes, the classical anionic peroxidases found in the xylem of poplar (Populus trichocarpa Trichobel) were purified and characterized. Five different poplar xylem peroxidases (PXP 1, PXP 2, PXP 3-4, PXP 5, and PXP (6) were isolated. All five peroxidases were strongly glycosylated (3.6% to 4.9% N-glucosamine), with apparent molecular masses between 46 and 54 kD and pI values between pH 3.1 and 3.8. Two of the five isolated peroxidases (PXP 3-4 and PXP (5) could oxidize the lignin monomer analog syringaldazine, an activity previously correlated with lignification in poplar. Because these isoenzymes were specifically or preferentially expressed in xylem, PXP 3-4 and PXP 5 are suggested to be involved in lignin polymerization
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0889eISSN: 1532-2548DOI: 10.1104/pp.118.1.125Titel-ID: cdi_proquest_miscellaneous_73873446
- Format
- –
- Schlagworte
- ACIDE COUMARIQUE, ACIDOS CUMARICOS, ACTIVIDAD ENZIMATICA, ACTIVITE ENZYMATIQUE, Agronomy. Soil science and plant productions, ALCOHOLES, ALCOHOLS, ALCOOL, Amino Acid Sequence, AMINO ACID SEQUENCES, Amino acids, Amino Acids - analysis, Biochemistry and Macromolecular Structure, Biological and medical sciences, BIOSINTESIS, BIOSYNTHESE, BIOSYNTHESIS, CELL WALLS, CHEMICAL COMPOSITION, CHEMICOPHYSICAL PROPERTIES, COMPOSICION QUIMICA, COMPOSITION CHIMIQUE, COUMARIC ACIDS, Economic plant physiology, Enzymes, ENZYMIC ACTIVITY, Fundamental and applied biological sciences. Psychology, Gels, Glycosylation, Isoelectric Point, ISOENZIMAS, ISOENZYME, ISOENZYMES, Isoenzymes - genetics, Isoenzymes - isolation & purification, Isoenzymes - metabolism, Lignification, Lignin, Lignin - biosynthesis, LIGNINAS, LIGNINE, LIGNINS, Metabolism, MOLECULAR SEQUENCE DATA, Molecular Weight, Nutrition. Photosynthesis. Respiration. Metabolism, OXIDACION, OXIDATION, OXYDATION, PARED CELULAR, PAROI CELLULAIRE, Peptide Fragments - genetics, Peptide Fragments - isolation & purification, PEROXIDASAS, PEROXIDASES, Peroxidases - genetics, Peroxidases - isolation & purification, Peroxidases - metabolism, PEROXYDASE, Plant physiology and development, Plants, POLIMERIZACION, POLYMERISATION, POLYMERIZATION, POPULUS TRICHOCARPA, PRECURSORS, PROPIEDADES FISICOQUIMICAS, PROPRIETE PHYSICOCHIMIQUE, PROTEINAS, PROTEINE, PROTEINS, PURIFICACION, PURIFICATION, Sequence Homology, Amino Acid, Substrate Specificity, SUBSTRATES, SYRINGALDAZINE, Trees - enzymology, Trees - genetics, Trees - metabolism, XILEMA, XYLEM, XYLEME