Immunity (Cambridge, Mass.), 1998-08, Vol.9 (2), p.267-276
1998
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Targeted Disruption of the Mouse Caspase 8 Gene Ablates Cell Death Induction by the TNF Receptors, Fas/Apo1, and DR3 and Is Lethal Prenatally
- Ist Teil von
- Immunity (Cambridge, Mass.), 1998-08, Vol.9 (2), p.267-276
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 1998
- Quelle
- EZB-FREE-00999 freely available EZB journals
- Beschreibungen/Notizen
- Homozygous targeted disruption of the mouse Caspase 8 ( Casp8) gene was found to be lethal in utero. The Caspase 8 null embryos exhibited impaired heart muscle development and congested accumulation of erythrocytes. Recovery of hematopoietic colony-forming cells from the embryos was very low. In fibroblast strains derived from these embryos, the TNF receptors, Fas/Apo1, and DR3 were able to activate the Jun N-terminal kinase and to trigger IκBα phosphorylation and degradation. They failed, however, to induce cell death, while doing so effectively in wild-type fibroblasts. These findings indicate that Caspase 8 plays a necessary and nonredundant role in death induction by several receptors of the TNF/NGF family and serves a vital role in embryonal development.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1074-7613eISSN: 1097-4180DOI: 10.1016/S1074-7613(00)80609-3Titel-ID: cdi_proquest_miscellaneous_73868529
- Format
- –
- Schlagworte
- Animals, Caspase 8, Caspase 9, Caspases, Cell Death - drug effects, Cell Death - genetics, Cells, Cultured - drug effects, Cysteine Endopeptidases - genetics, Cysteine Endopeptidases - physiology, DNA, Complementary - genetics, fas Receptor - pharmacology, fas Receptor - physiology, Fetal Death - genetics, Fibroblasts - cytology, Fibroblasts - drug effects, Fibroblasts - physiology, Gene Targeting, Genes, Lethal - genetics, Gestational Age, Mice, Mice, Inbred C57BL, Mice, Knockout - embryology, Phenotype, Receptors, Tumor Necrosis Factor - physiology, Receptors, Tumor Necrosis Factor, Member 25, Transcription, Genetic - genetics