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Extractant effects on some properties of rhodopsin
Ist Teil von
Vision research (Oxford), 1977, Vol.17 (10), p.1157-1162
Ort / Verlag
England: Elsevier Ltd
Erscheinungsjahr
1977
Link zum Volltext
Quelle
Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
Beschreibungen/Notizen
The photosensitivity, thermal stability and rate of metarhodopsin II production of rhodopsin solubilized in five commonly used extracting agents have been determined and compared with rod outer segment suspensions. An effect of extracting agent on photosensitivity was found, indicating that the electronic interactions of the chromophore and protein are sensitive to the micellar environment. Both the rate of thermal decay and metarhodopsin II production were found to be a function of the detergent. Only digitonìn gave rates which were comparable to those seen in rod outer segment suspensions.