Details

Autor(en) / Beteiligte

• Maitland, Norman J
• Kinross, John H
• Busuttil, Anthony
• Ludgate, Suzanne M
• Smart, George E
• Jones, Kenneth W

Titel

The Detection of DNA Tumour Virus-specific RNA Sequences in Abnormal Human Cervical Biopsies by in situ Hybridization

Ist Teil von

• Journal of general virology, 1981-07, Vol.55 (1), p.123-137

Ort / Verlag

England: Soc General Microbiol

Erscheinungsjahr

1981

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• 1 Institute of Animal Genetics, University of Edinburgh, Edinburgh 2 Department of Pathology, Western General Hospital, Edinburgh 3 Department of Radiotherapy, Western General Hospital, Edinburgh 4 Department of Obstetrics and Gynaecology, Royal Infirmary, Edinburgh, U.K. We have used the technique of in situ nucleic acid hybridization and autoradiography of thin frozen sections of human tissue to search for virus RNA sequences in human cervical tumours. Of cervical biopsies with abnormal cytology, 67% bound herpes simplex virus type 2 (HSV2) 3 H-labelled DNA probes and 39% bound adenovirus type 2 (Ad2) 3 H-labelled DNA probes, whereas control experiments with phage lambda 3 H-labelled DNA probes, under the same conditions, bound to only 7% of cases. In contrast, normal cervical biopsies bound the three probes in only 23%, 17% and 8% of cases respectively. Keywords: herpes simplex, adenovirus, tumour virus, RNA sequence Received 11 November 1980; accepted 10 February 1981.