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Complete photosystem I from a higher plant (pea; Pisum sativum) was isolated, purified and crystallized. The crystals diffract to 4 Å resolution using synchrotron radiation and belong to the monoclinic crystal system, space group P21, with unit‐cell parameters a = 181.90, b = 190.24, c = 219.66 Å, β = 90.484°. Data sets from three crystals have been collected and were merged into a 4.4 Å resolution data set, taking advantage of the high isomorphicity observed for these crystals. Analysis of the self‐rotation Patterson function suggests the possible presence of two PSI complexes in the asymmetric unit.