Details

Autor(en) / Beteiligte

• TAKEUCHI, Yutaka
• YOSHIZAKI, Goro
• TAKEUCHI, Toshio

Titel

Generation of Live Fry from Intraperitoneally Transplanted Primordial Germ Cells in Rainbow Trout

Ist Teil von

• Biology of reproduction, 2003-10, Vol.69 (4), p.1142-1149

Ort / Verlag

Madison, WI: Society for the Study of Reproduction

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• Germ cell transplantation has tremendous applications in transgenic animal production, assisted reproductive technology, and germline stem cell research. Here, we report for the first time the production of individuals from intraperitoneally transplanted primordial germ cells (PGCs) in animals. To trace the behavior of exogenous PGCs in recipients, PGCs visualized by a green fluorescent protein gene were used as donors. The PGCs prepared from the genital ridges of hatching embryos were transplanted into recipients at various developmental stages. The PGCs injected into the peritoneal cavities of hatching embryos had the ability to migrate toward, and to colonize, the genital ridges of recipient embryos. Furthermore, donor-derived PGCs proliferated and differentiated into mature eggs and sperm in the allogenic gonads; the resulting gametes produced live fry, showing the donor-derived phenotype, through fertilization. Combined with in vitro culture, genetic modification, and cryopreservation of PGCs, this technique provides new approaches for fish bioengineering.