UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 9 von 89
Datensatz exportieren als...
BibTeX
Intravenous bone marrow stromal cell therapy reduces apoptosis and promotes endogenous cell proliferation after stroke in female rat
Journal of neuroscience research, 2003-09, Vol.73 (6), p.778-786
Chen, Jieli
Li, Yi
Katakowski, Mark
Chen, Xiaoguang
Wang, Lei
Lu, Dunyue
Lu, Mei
Gautam, Subhash C.
Chopp, Michael
2003
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Chen, Jieli
Li, Yi
Katakowski, Mark
Chen, Xiaoguang
Wang, Lei
Lu, Dunyue
Lu, Mei
Gautam, Subhash C.
Chopp, Michael
Titel
Intravenous bone marrow stromal cell therapy reduces apoptosis and promotes endogenous cell proliferation after stroke in female rat
Ist Teil von
Journal of neuroscience research, 2003-09, Vol.73 (6), p.778-786
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2003
Quelle
Access via Wiley Online Library
Beschreibungen/Notizen
The present study investigates the induction of neurogenesis, reduction of apoptosis, and promotion of basic fibroblast growth factor (bFGF) expression as possible mechanisms by which treatment of stroke with bone marrow stromal cells (MSCs) improves neurological functional recovery. Additionally, for the first time, we treated cerebral ischemia in female rats with intraveneous administration of MSCs. Female rats were subjected to 2 hr of middle cerebral artery occlusion (MCAo), followed by an injection of 3 × 106 male (for Y chromosome labeling) rat MSCs or phosphate‐buffered saline (PBS) into the tail vein 24 hr after MCAo. All animals received daily injection of bromodeoxyuridine (BrdU; 50 mg/kg, i.p.) for 13 days after treatment for identification of newly synthesized DNA. Animals were sacrificed at 14 days after MCAo. Behavioral tests (rotarod and adhesive‐removal tests) were performed. In situ hybridization, immunohistochemistry, and terminal deoxynucleotidyltransferase (TdT)‐mediated dUTP‐biotin nick‐end labeling (TUNEL) were performed to identify transplanted MSCs (Y chromosome), BrdU, bFGF, and apoptotic cells in the brain. Significant recovery of behavior was found in MSC‐treated rats at 7 days in the somatosensory test and at 14 days in the motor test after MCAo compared with control, PBS‐treated animals (P < .05). MSCs were found to survive and preferentially localize to the ipsilateral ischemic hemisphere. Significantly more BrdU‐positive cells were located in the subventricular zone (P < .05), and significantly fewer apoptotic cells and more bFGF immunoreactive cell were found in the ischemic boundary area (P < .05) of MSC‐treated rats than in PBS‐treated animals. Here we demonstrate that intravenously administered male MSCs increase bFGF expression, reduce apoptosis, promote endogenous cellular proliferation, and improve functional recovery after stroke in female rats. © 2003 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0360-4012
eISSN: 1097-4547
DOI: 10.1002/jnr.10691
Titel-ID: cdi_proquest_miscellaneous_73611171
Format
–
Schlagworte
Animals
,
Apoptosis
,
apoptosis, female rat
,
Behavior, Animal
,
Bone Marrow Transplantation
,
Bromodeoxyuridine - metabolism
,
Cell Count
,
Cell Division
,
Cells, Cultured
,
cerebral ischemia
,
Disease Models, Animal
,
Female
,
female rat
,
Fibroblast Growth Factor 2 - metabolism
,
Glial Fibrillary Acidic Protein - metabolism
,
Immunohistochemistry
,
In Situ Hybridization
,
In Situ Nick-End Labeling
,
Infarction, Middle Cerebral Artery - physiopathology
,
Infarction, Middle Cerebral Artery - therapy
,
marrow stromal cells
,
MCAO
,
Motor Activity
,
Nerve Tissue Proteins - metabolism
,
Nuclear Proteins - metabolism
,
Psychomotor Performance - physiology
,
Rats
,
Rats, Wistar
,
Reaction Time
,
Recovery of Function
,
Stromal Cells - metabolism
,
Stromal Cells - transplantation
,
subventricular zone
,
Time Factors
,
Transplantation
,
Tubulin - metabolism
,
von Willebrand Factor - metabolism
,
Y Chromosome - metabolism
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX