Details

Autor(en) / Beteiligte

• Jay S. Morrow
• Bruce C. Weintraub
• Rosen, Saul W.

Titel

Ectopic Production in Serum-Free Media of the Common Alpha Subunit of the Glycoprotein Hormones

Ist Teil von

• In Vitro, 1981-05, Vol.17 (5), p.421-426

Ort / Verlag

United States: Tissue Culture Association, Inc

Erscheinungsjahr

1981

Quelle

MEDLINE

Beschreibungen/Notizen

• The$HeLa-S_3$cell strain grown in Ham's F12 medium supplemented with insulin, transferrin, cortisol, epidermal growth factor, fibroblast growth factor, and trace elements, but containing no serum, continued to produce the common$\alpha-subunit$of the glycoprotein hormones for the 10 d study. The amounts of$\alpha-subunit$secreted into the medium during the first 4 d were indistinguishable from those in F12 medium supplemented with 10% fetal bovine serum. During the remainder of the experiment the amounts of$\alpha-subunit$reached 50 to 80% those in the serum-supplemented medium.