Details

Autor(en) / Beteiligte

• Poncet, S.
• Gasc, J.-M.
• Janzer, R. C.
• Meyer, S.
• Juillerat-Jeanneret, L.

Titel

Expression of Tie-2 in human peripheral and autonomic nervous system

Ist Teil von

• Neuropathology and applied neurobiology, 2003-08, Vol.29 (4), p.361-369

Ort / Verlag

Oxford, UK: Blackwell Science Ltd

Erscheinungsjahr

2003

Quelle

Wiley Online Library All Journals

Beschreibungen/Notizen

• Tie‐2, a tyrosine kinase receptor, is essential for vascular integrity by regulating cellular adhesion between pericytes and endothelial cells. The aim of this study was to identify sites of expression of Tie‐2 other than the vasculature. Tie‐2 expression was first detected in human colon by Western blotting and reverse‐transcription‐polymerase chain reaction (RT‐PCR) in tissue extracts. The presence of the Tie‐2 mRNA and protein was detected by immunohistochemistry and in situ hybridization in cells of the colon myenteric and submucosal plexus, in both neuronal and Schwann cells. Tie‐2 protein was also found in the nervous system of the female urogenital tract. In the human sciatic nerve and schwannoma, RT‐PCR, Western blotting and immunohistochemistry analysis further confirmed the presence of Tie‐2 mRNA and protein in non‐autonomic peripheral nervous tissue. In conclusion, using several approaches and tissues we have demonstrated the presence of Tie‐2 in human peripheral and autonomic nervous tissue, suggesting a role for Tie‐2 in neural tissue. Thus, attempts to disrupt the tumour vessels by manipulation of the Tie‐2 system in tumours may result in side‐effects in peripheral nerves.