Details

Autor(en) / Beteiligte

• Torti, Carlo
• Quiros-Roldan, Eugenia
• Keulen, Wilco
• Scudeller, Luigia
• Lo Caputo, Sergio
• Boucher, Charles
• Castelli, Francesco
• Mazzotta, Francesco
• Pierotti, Piera
• Been-Tiktak, Anne Mieke
• Buccoliero, Giovanni
• De Gennaro, Michele
• Carosi, Giampiero
• Tinelli, Carmine

Titel

Comparison between Rules-Based Human Immunodeficiency Virus Type 1 Genotype Interpretations and Real or Virtual Phenotype: Concordance Analysis and Correlation with Clinical Outcome in Heavily Treated Patients

Ist Teil von

• The Journal of infectious diseases, 2003-07, Vol.188 (2), p.194-201

Ort / Verlag

Chicago, IL: The University of Chicago Press

Erscheinungsjahr

2003

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• We compared 2 rules-based genotype interpretation systems and real or virtual phenotype through a retrospective analysis of a prospective trial. Genotypes were determined with VircoGEN II (VIRCO) and were interpreted with either RetroGram 1.4 or TRUGENE HIV-1 (guidelines 3.0) or original virtual phenotype (Virtual Phenotype; VIRCO), as available in the year 2000. Among 188 human immunodeficiency virus (HIV) type 1 isolates, overall concordance (κ agreement) was observed for the 2 rules-based systems, whereas striking discordances were noted between them and real and virtual phenotype interpretations for stavudine, didanosine, zalcitabine, abacavir, and amprenavir (κ<0.4). Clinical evaluation of a subset of 173 patients showed that both rules-based sensitivity scores were independently associated with HIV RNA loads <400 copies/mL at week 16 of during-treatment analysis (TRUGENE: odds ratio [OR], 2.90; 95% confidence interval [CI], 1.52–5.52; P = .001 ; RetroGram: OR, 2.34; 95% CI, 1.21–4.55; P = .012), whereas, in contrast to real or virtual phenotype, interpretations according to biological cut-offs were not (OR, 1.91; 95% CI, 0.77–4.76; P = .162).